Osteoblast biocompatibility and inhibition of bacterial adhesion to thermally and chemically treated TiAlV alloy

Authors

  • Greta Tavarez-Martínez National Polytechnic Institute, Centro de Investigación Ciencia Aplicada y Tecnología Avanzada, Unidad Altamira, Km 14.5 Carretera Tampico-Puerto Industrial, 89600 Altamira, Tamaulipas https://orcid.org/0000-0002-2625-3052
  • Belén Criado Spanish National Research Council, National Center for Metallurgical Research (CSIC, CENIM), Department of Surface Engineering, Corrosion and Durability, Avda. Gregorio del Amo 8, 28040 Madrid https://orcid.org/0000-0002-5486-9604
  • M. Coronada Fernández-Calderón Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine Network (CIBER-BBN), Badajoz. University of Extremadura, Faculty of Medicine and INUBE, Department of Biomedical Sciences, Area of Microbiology https://orcid.org/0000-0001-8567-6787
  • Edgar Onofre-Bustamante National Polytechnic Institute, Centro de Investigación Ciencia Aplicada y Tecnología Avanzada, Unidad Altamira, Km 14.5 Carretera Tampico-Puerto Industrial, 89600 Altamira, Tamaulipas https://orcid.org/0000-0002-5706-887X
  • Ciro Pérez-Giraldo Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine Network (CIBER-BBN), Badajoz. University of Extremadura, Faculty of Medicine and INUBE, Department of Biomedical Sciences, Area of Microbiology https://orcid.org/0000-0002-2358-1982
  • Cristina García-Alonso Spanish National Research Council, National Center for Metallurgical Research (CSIC, CENIM), Department of Surface Engineering, Corrosion and Durability, Avda. Gregorio del Amo 8, 28040 Madrid https://orcid.org/0000-0003-0275-4626
  • Mª Lorenza Escudero Spanish National Research Council, National Center for Metallurgical Research (CSIC, CENIM), Department of Surface Engineering, Corrosion and Durability, Avda. Gregorio del Amo 8, 28040 Madrid https://orcid.org/0000-0002-2181-448X

DOI:

https://doi.org/10.3989/revmetalm.208

Keywords:

Nanoceria deposition, Osteoblasts MC3T3-E1, Staphylococcus epidermis, TiAlV, Thermal treatment

Abstract


The objective of this work was to study whether thermal and chemical conversion treatments improve the biocompatibility of the TiAlV alloy and reduce bacterial growth. Firstly, TiAlV alloy was modified by thermal treatment at 650 ºC for 1 hour. Then, chemical conversion was carried out in a CeCl3 solution to generate cerium oxide. Modified surfaces were characterized using AFM and SEM-EDX. Osteoblast adhesion and bacteria biofilm formation were measured in vitro with MC3T3-E1 osteoblast cell line and Staphylococcus epidermidis ATCC 35983, respectively. Bacterial viability was quantified through content in adenosine triphosphate (ATP) as a measure of metabolic activity. Morphology and proliferation on modified surfaces were analyzed by SEM-EDX. Results revealed that thermally treated TiAlV showed greater osteoblast proliferation viability associated with greater roughness and crystalline structure of rutile. Modified surfaces did not cause bactericidal effect but TiAlV surfaces with ceria showed a decrease in bacterial adhesion i.e. less bacteria proliferation and therefore a decrease in bacterial colonization.

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Published

2021-12-30

How to Cite

Tavarez-Martínez, G., Criado, B., Coronada Fernández-Calderón, M., Onofre-Bustamante, E., Pérez-Giraldo, C., García-Alonso, C., & Lorenza Escudero, M. (2021). Osteoblast biocompatibility and inhibition of bacterial adhesion to thermally and chemically treated TiAlV alloy. Revista De Metalurgia, 57(4), e208. https://doi.org/10.3989/revmetalm.208

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Articles

Funding data

Consejo Nacional de Ciencia y Tecnología, Paraguay
Grant numbers 2012 #183416

Ministerio de Economía y Competitividad
Grant numbers MAT2015-63974-C4-4-R

Ministerio de Ciencia e Innovación
Grant numbers RTI2018-101506-B-C31

Junta de Extremadura
Grant numbers GR18096