Biocompatibilidad de osteoblastos e inhibición de adhesión bacteriana a la aleación Ti6Al4V tratada térmica y químicamente

Greta Tavarez-Martínez; Belén Criado; M. Coronada Fernández-Calderón; Edgar Onofre-Bustamante; Ciro Pérez-Giraldo; Cristina García-Alonso; Mª Lorenza Escudero

doi:10.3989/revmetalm.208

Autores/as

Greta Tavarez-Martínez National Polytechnic Institute, Centro de Investigación Ciencia Aplicada y Tecnología Avanzada, Unidad Altamira, Km 14.5 Carretera Tampico-Puerto Industrial, 89600 Altamira, Tamaulipas https://orcid.org/0000-0002-2625-3052
Belén Criado Spanish National Research Council, National Center for Metallurgical Research (CSIC, CENIM), Department of Surface Engineering, Corrosion and Durability, Avda. Gregorio del Amo 8, 28040 Madrid https://orcid.org/0000-0002-5486-9604
M. Coronada Fernández-Calderón Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine Network (CIBER-BBN), Badajoz. University of Extremadura, Faculty of Medicine and INUBE, Department of Biomedical Sciences, Area of Microbiology https://orcid.org/0000-0001-8567-6787
Edgar Onofre-Bustamante National Polytechnic Institute, Centro de Investigación Ciencia Aplicada y Tecnología Avanzada, Unidad Altamira, Km 14.5 Carretera Tampico-Puerto Industrial, 89600 Altamira, Tamaulipas https://orcid.org/0000-0002-5706-887X
Ciro Pérez-Giraldo Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine Network (CIBER-BBN), Badajoz. University of Extremadura, Faculty of Medicine and INUBE, Department of Biomedical Sciences, Area of Microbiology https://orcid.org/0000-0002-2358-1982
Cristina García-Alonso Spanish National Research Council, National Center for Metallurgical Research (CSIC, CENIM), Department of Surface Engineering, Corrosion and Durability, Avda. Gregorio del Amo 8, 28040 Madrid https://orcid.org/0000-0003-0275-4626
Mª Lorenza Escudero Spanish National Research Council, National Center for Metallurgical Research (CSIC, CENIM), Department of Surface Engineering, Corrosion and Durability, Avda. Gregorio del Amo 8, 28040 Madrid https://orcid.org/0000-0002-2181-448X

DOI:

https://doi.org/10.3989/revmetalm.208

Palabras clave:

Depósito de nanoceria, Osteoblastos MC3T3-E1, Staphylococcus epidermis, TiAlV, Tratamiento térmico

Resumen

El objetivo de este trabajo ha sido estudiar si los tratamientos térmicos y de conversión química mejoran la biocompatibilidad de la aleación TiAlV y reducen el crecimiento bacteriano. En primer lugar, se modificó la aleación de TiAlV mediante tratamiento térmico a 650 ºC durante 1 hour. Luego, se llevó a cabo la conversión química en una solución de CeCl3 para generar óxido de cerio. Las superficies modificadas se caracterizaron utilizando AFM y SEM-EDX. La adhesión de osteoblastos y la formación de biopelículas microbianas se midieron in vitro con la línea celular de osteoblastos MC3T3-E1 y Staphylococcus epidermidis ATCC 35983, respectivamente. La viabilidad bacteriana se cuantificó a través del contenido en trifosfato de adenosina (ATP) como medida de la actividad metabólica. La morfología y la proliferación en superficies modificadas se analizaron mediante SEM-EDX. Los resultados revelaron que el TiAlV tratado térmicamente mostró una mayor proliferación osteoblástica asociada con una mayor rugosidad y estructura cristalina del rutilo. Las superficies modificadas no causaron efecto bactericida, pero las superficies de TiAlV con ceria mostraron una disminución en la adhesión bacteriana, es decir, menos proliferación bacteriana y por tanto disminución en la colonización bacteriana.

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