Influence of Ag nanoparticles on the mechanical and tribological properties and on the cytotoxic and bactericidal effects of TaN(Ag) coatings




Ag nanoparticles, Bactericidal effect, Biocompatible coatings, Citotoxicity, Magnetron sputtering


In this work, a TaN(Ag) composite coating with different silver contents between 2.26 and 28.51 %at, were developed by the unbalanced magnetron sputtering technique. The coating with the best balance presented between tribological and mechanical properties was chosen, and it was subjected to thermal treatments cycles with temperatures among 175 °C and 275 °C to allow the nucleation, growth and controlled diffusion of Ag nanoparticles up to the coating surface; the size, distribution and density of nanoparticles on the coating surface and their influence on the mechanical and tribological properties, were verified. The bactericidal effect against P. aeruginosa by growth inhibition and adhesion test was studied, as well as the cytotoxic behavior on osteoblasts by MTT test. The TaN(Ag)-3 coating after thermal treatment at 200 °C during 4 min, increased the micro hardness of AISI 316L stainless steel from 2.6 GPa to 10.7GPa, and decreased the wear rate from 1.11x10-3 mm3/N.m to 0.17x10-12 mm3/N.m. This coating exhibited 100% of osteoblast cell viability, and an excellent performance to the inhibition of growth and adherence of P. aeruginosa.


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Baghriche, O., Kiwi, J., Pulgarin, C., Sanjinés, R. (2012). Antibacterial Ag–ZrN surfaces promoted by subnanometric ZrN-Clusters deposited by reactive pulsed magnetron sputtering. J. Photoch. Photobio. A 229 (1), 39–45.

Bai, Y., Yan, L., Wang, J., Zhigang, Y., Nuofu, Ch., Fuzhi, W., Zhan'ao, T. (2016). Tailoring Film Agglomeration for Preparation of Silver Nanoparticles with Controlled Morphology. Mater. Design 103, 315–320.

Bhatnagar, M., Mukesh, R., Jolley, K., Lloyd, A., Smith, R., Mukherjee, S. (2017). Thermal dynamics of silver clusters grown on rippled silica surfaces. Nucl. Instrum. Meth. B 393, 5–12.

Bowker, M. (2009). A Prospective: Surface Science and Catalysis at the Nanoscale. Surf. Sci. 603 (16), 2359–2362.

Chang, Y., Chih-Ho, L., Hsu, J.T., Tang, Ch.H., Liao, W.Ch., Huang, H.L (2012). Antibacterial Properties and Human Gingival Fibroblast Cell Compatibility of TiO2/Ag Compound Coatings and ZnO Films on Titanium-Based Material. Clin. Oral Investig. 16 (1), 95–100. PMid:21234622

Chernousova, S., Epple, M. (2013). Silver as Antibacterial Agent: Ion, Nanoparticle, and Metal. Angew. Chem-Ger. Edit. 52 (6), 1636–1653. PMid:23255416

Echeverrigaray, F.G., Echeverrigaray, S., Delamare, A.P.L., Wanke, C.H., Figueroa, C.A., Baumvol, I.J.R., Aguzzoli, C. (2016). Antibacterial Properties Obtained by Low-Energy Silver Implantation in Stainless Steel Surfaces. Surf. Coat. Tech. 307 (Part A), 345–351.

Falconer, J.L., Grainger, D.W. (2017). Silver Antimicrobial Biomaterials. In Reference Module in Mat. Sci. Mater. Eng.

Gopinath, V., Velusamy, P. (2013). Extracellular biosynthesis of silver nanoparticles using bacillus Sp. GP-23 and evaluation of their antifungal activity towards Fusarium oxysporum. Spectrochim. Acta A–M 106, 170–174. PMid:23376272

Jiang, Y., Yin, Y.J., Zha, X.Ch., Dou, X.Q., Feng, Ch.L. (2016). Wettability Regulated Gram-Negative Bacterial Adhesion on Biomimetic Hierarchical Structures. Chinese Chem. Lett. 5–9.

Liskiewicz, T., Fouvry, S., Wendler, B. (2005). Development of a Wohler-like approach to quantify the Ti(CxNy) coatings durability under oscillating sliding conditions. Wear 259 (7–12), 835–841.

Liu, X., Iamvasant, C., Liu, C., Matthews, A., Leyland, A. (2017). CrCuAgN PVD Nanocomposite Coatings: Effects of Annealing on Coating Morphology and Nanostructure. Appl. Surf. Sci. 392, 732–746.

Madhuree, K., Pandey, S., Giri, V.P., Bhattacharya, A., Shukla, R., Mishra, A., Nautiyal, C.S. (2016). Tailoring Shape and Size of Biogenic Silver Nanoparticles to Enhance Antimicrobial Efficacy against MDR Bacteria. (In Press) Microb. Pathogenesis.

Manninen, N.K., Escobar G.R, Benito, N., Figueiredo, N.M., Cavaleiro, A., Palacio, C., Carvalho, S. (2011). Ag-Ti (C,N) Based Coatings for Biomedical Applications : Influence of Silver Content on the Structural Properties. J. Phys. D Appl. Phys. 44 (37), 375501.

Marques, S.M., Rico, P., Carvalho, I., Gómez Ribelles, J.L., Fialho, L., Lanceros-Méndez, S., Henriques, M., Carvalho, S. (2016). MC3T3-E1 Cell Response to Ti 1– X Ag X and Ag-TiN X Electrodes Deposited on Piezoelectric Poly(vinylidene Fluoride) Substrates for Sensor Applications. Appl. Mater. Interfaces 8 (6), 4199–4207. PMid:26840928

Misra, S.K., Dybowska, A., Berhanu, D., Luoma, S.N., Valsami-Jones, E. (2012). The complexity of nanoparticle dissolution and its importance in nanotoxicological studies. Sci. Total Environ. 438, 225–232. PMid:23000548

Morones-Ramirez, J.R., Winkler, J.A., Spina, C.S., Collins. J. J. (2013). Silver Enhances Antibiotic Activity Against Gram-Negative Bacteria. Sci. Transl. Med. 5 (190), 1–11.

Muratore, C., Hu, J.J., Voevodin, A.A. (2007). Adaptive nanocomposite coatings with a titanium nitride diffusion barrier mask for high-temperature tribological applications. Thin Solid Films 515 (7–8), 3638–3643.

Rycenga, M., Cobley, C.M., Zeng, J., Weiyang, L., Moran, C.H., Zhang, Q., Qin, D., Xia, Y. (2011). Controlling the Synthesis and Assembly of Silver Nanostructures for Plasmonic Applications. Chem. Rev. 111 (6), 3669–3712. PMid:21395318 PMCid:PMC3110991

Tallósy, S.P., Janovák, L., Nagy, E., Deák, A., Juhász, A., Csapó, E., Buzás, N., Dékány, I. (2016). Adhesion and Inactivation of Gram-Negative and Gram-Positive Bacteria on Photoreactive TiO2/polymer and Ag-TiO2/polymer Nanohybrid Films. Appl. Surf. Sci. 371, 139–150.

Taoyu, W., Stylios, G.K., Giannoudi, M., Giannoudis, P.V. (2015). Investigating a New Drug Delivery Nano Composite Membrane System Based on PVA/PCL and PVA/HA(PEG) for the Controlled Release of Biopharmaceuticals for Bone Infections. Injury 46, 39–43.

Tianlu, Z., Wang, L., Chen, Q., Chen, Ch. (2014). Cytotoxic Potential of Silver Nanoparticles. Yonsei Med. J. 55, 283–291. PMid:24532494 PMCid:PMC3936614

Truong, V.K., Lapovok, R., Estrin, Y.S., Stuart Rundell, J., Wang, Y., Fluke, C.J, Crawford, R.J., Ivanova, E.P. (2010). The influence of nano-scale surface roughness on bacterial adhesion to ultrafine-grained titanium. Biomaterials 31 (13), 3674–3683. PMid:20163851

Wang, J., Kaplan, J.A., Colson, Y.L., Grinstaff, M.W. (2016). Mechanoresponsive materials for drug delivery: harnessing forces for controlled release. Adv. Drug Deliver. Rev. 108, 68–82. PMid:27856307

Wei, Z., Chu, P.K. (2008). Enhancement of Antibacterial Properties and Biocompatibility of Polyethylene by Silver and Copper Plasma Immersion Ion Implantation. Surf. Coat. Tech. 203, 909–912.



How to Cite

Echavarría, A. M., Robledo, S., & Bejarano G., G. (2017). Influence of Ag nanoparticles on the mechanical and tribological properties and on the cytotoxic and bactericidal effects of TaN(Ag) coatings. Revista De Metalurgia, 53(1), e085.