Análisis bioquímico del micelio del hongo <i>Hormoconis resinae</i> en la corrosión de aleaciones aeronáuticas de aluminio

R. Araya; C. Bobadilla; R. Vera; B. M. Rosales

doi:10.3989/revmetalm.2007.v43.i3.68

Authors

R. Araya Laboratorio de Corrosión, Instituto de Química, Pontificia Universidad Católica de Valparaíso
C. Bobadilla Laboratorio de Corrosión, Instituto de Química, Pontificia Universidad Católica de Valparaíso
R. Vera Laboratorio de Corrosión, Instituto de Química, Pontificia Universidad Católica de Valparaíso
B. M. Rosales CIDEPINT

DOI:

https://doi.org/10.3989/revmetalm.2007.v43.i3.68

Keywords:

Hormoconis resinae, MIC, Aeronautic, Aluminium alloys, Biochemical analyses

Abstract

Biochemical analyses of the Hormoconis resinae fungal mycelium would explain behaviour differences of corrosive and non-corrosive strains on Al and its aeronautical alloys. In previous works its aggressiveness had been studied through SEM-EDX surface analysis, electrochemical techniques and immersion testing. In this paper separation of the proteins of the mycelium produced by a non-corrosive strain and its culture along three generations was performed. Cultures were prepared in batch in the presence and absence of pure Al and AA 2024, AA 7005 and AA 7075 alloys. The mycelia grown throughout the three generations increasingly
recovered usual characteristics at the third replication, included their corrosiveness on Al and its alloys previously shown by all our strains. Amongst the bio-molecule fractions isolated and analysed during this preliminary study only the proteins revealed changes with the generation grown. When this fungal strain was cultured in the presence of alloy metal sheets electrophoresis of the protean fraction was correlative with the distinct mycelia behaviour observed, including corrosiveness on Al and its alloys.

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References

[1] D.G. Parbery, Mater. Org. 6 (1971) 161-208

[2] D.G. Parbery, Biodeterioration of metals, Applied Science Publishers Ltd., England, 1972, pp. 65-74.

[3] P. Mc Kenzie, A.S. Akbar and J.D. Miller, Institute of Petroleum (Technical paper), 1977, pp. 37-50.

[4] D. Cabral, Int. Biodeterioration Bull. 16 (1980) 23-27.

[5] J.D. Bu’lock, J.E. Smith and D.R. Berry, Eds., Industrial Mycology, Pub. Edward Arnold (UK), 1975, pp. 33-57.

[6] B.M. Rosales, S. Chichizola and D. BaleanI, Proc. 1st NACE Latin American Region Corros. Cong. and 1st Venezuelan Corros. Cong., 2, paper 94054, 1994, pp. 1-11.

[7] B.M. Rosales, E.S. Ayllón and M.C. Leiro, New Methods for Corrosion Testing of Aluminium Alloys, ASTM STP 1134, Agarwala, V. S., Ugiansky, G. M. Eds. ASTM, Philadelphia, USA, 1992, pp. 50-59.

[8] B.M. Rosales and E.R. De Schiapparelli, Mater. Perform. 19 (1980) 41-44.

[9] E.R. De Schiapparelli and B.M. Rosales, Mater. Perform. 19 (1980) 47-50.

[10] O.A. Biscione, E.S. Ayllón and B. M.Rosales, Int. Biodeterioration 23 (1987) 159-166. doi:10.1016/0265-3036(87)90051-0

[11] E.S. Ayllón and B.M. Rosales, Corrosion NACE 44 (1988) 638-43.

[12] M. Iannuzzi, G. Duffó and B.M.Rosales. Proc. 15th Int. Corros. Cong., Paper 77, Granada, Spain, 2002.

[13] M. Iannuzzi and B.M. Rosales. Materi. Perform. J. NACE (2003) 62-66.

[14] B.M. Rosales, A.C. Cabezas, S.E. Chichizola and A.Fernández, 3rd Latin American Biodegradation & Biodeterioration Symp., Florianópolis, Brazil, paper 78, 1998.

[15]A.M. Palermo, S. Chichizola and B.M. Rosales, Proc. Second Latin American Biodegradation and Biodeterioration Symp., Gramado, Brasil, 1995.

[16] E.S. Ayllon, B.M. Rosales, Corrosion NACE, 50, Nº 8 (1994) 571-575.

[17] B.M. Rosales, Proc. 11th Int. Corrosion Cong., Florence, Italy, 4, 1990, p. 359-366.

[18] B.M. Rosales, A. Puebla, D. Cabral, Proc. XI Int. Corros. Cong., Houston, USA, 5B, 1993, pp. 3773-3785.

[19] M.I. Brown and J.N. Lester, Water Res. 13 (1979) 817-837. doi:10.1016/0043-1354(79)90217-3

[20] D.E. Klemme and R. Neihof, Memorandum Report 3212, USA, (1976) pp. 1-15.

[21] L.D. Bushnell and H.F. Haas, Bacteriology 41 (1941) 653-673.

[22] J.C. Janson, L. Ryden, In protein purification. VCH Publishers, 1989, pp. 233-267.

[23] U.K. Laemmli, Nature 227 (1970) 680-685. doi:10.1038/227680a0

PMid:5432063

[24] O.H. Lowry, N.J. Roseborough, A.L. Fall, R.J. Randall, J.Biol. Chem. 193 (1951) 265-275.

[25] M.M. Bradford, Anal. Biochem. 72 (1976) 248-254. doi:10.1016/0003-2697(76)90527-3

PMid:942051

[26] A. Rajasekar, S. Marythamuthu, N. Muthukumar, S. Mohanan, P. Subramanian and N. Palaniswamy, Corros. Sci. 47 (2005) 257-271. doi:10.1016/j.corsci.2004.05.016

[27] D.J. Hansen, D.J. Tighe-Ford and G.C. George, Int. Biodeterioration Bull. 17 (1981) 103-112.

[28] V. Zinkevich, I. Bogdarina, H. Kang, M.A.W. Hill, R. Tapper, and I.B. Beech, Int. Biodeterioration Biodegradation 37 (1996) 163-172. doi:10.1016/S0964-8305(96)00025-X

[29] I.B. Beech and C.W.S. Cheung Int. Biodeterioration Biodegradation 35 (1995) 59-72. doi:10.1016/0964-8305(95)00082-G

[30] B. Rosales and E.R De Schiapparelli, Int. Biodeterioration Bull., 16 Summer (1980) 313 6 .