Adhesión de osteoblastos sobre una superficie de Ti de rugosidad controlada

Laura Burgos-Asperilla; M. Cristina García-Alonso; M. Lorenza Escudero; Concepción Alonso

doi:10.3989/revmetalm.044

Autores/as

Laura Burgos-Asperilla Department of Applied Physical Chemistry, Universidad Autónoma de Madrid
M. Cristina García-Alonso Department of Applied Physical Chemistry, Universidad Autónoma de Madrid - Department of Surface Engineering, Corrosion and Durability, National Centre for Metallurgical Research (CENIM-CSIC)
M. Lorenza Escudero Department of Applied Physical Chemistry, Universidad Autónoma de Madrid - Department of Surface Engineering, Corrosion and Durability, National Centre for Metallurgical Research (CENIM-CSIC)
Concepción Alonso Department of Applied Physical Chemistry, Universidad Autónoma de Madrid

DOI:

https://doi.org/10.3989/revmetalm.044

Palabras clave:

Espectroscopia de impedancia electroquímica, Microbalanza de cristal de cuarzo, Osteoblastos Saos-2, Titanio

Resumen

En este trabajo, se ha estudiado la interacción in situ entre células osteoblásticas Saos-2 y una superficie de Ti de rugosidad controlada a lo largo del tiempo. El estudio de la cinética y los mecanismos de proliferación celular de adhesión se ha realizado a través de la microbalanza de cristal de cuarzo (QCM) y espectroscopía de impedancia electroquímica (EIS). La velocidad de adhesión de los osteoblastos sobre la superficie de Ti obtenida a través de medidas con la QCM, sigue una reacción de primer orden, con k=2×10⁻³ min⁻¹. Los ensayos de impedancia indican que, en ausencia de las células, la resistencia del Ti disminuye con el tiempo (7 días), debido a la presencia de aminoácidos y proteínas del medio de cultivo que se han adsorbido, mientras que en presencia de células, esta disminución es mucho mayor debido a los productos metabólicos generados por las células que aceleran la disolución del Ti.

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